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dc.contributor.authorGederaas, Odrun Arna
dc.contributor.authorSharma, Animesh
dc.contributor.authorMbarak, Saide
dc.contributor.authorSporsheim, Bjørnar
dc.contributor.authorHøgset, Anders
dc.contributor.authorBogoeva, Vanya
dc.contributor.authorSlupphaug, Geir
dc.contributor.authorHagen, Lars
dc.identifier.citationGederaas, O. A., Sharma, A., Mbarak, S., Sporsheim, B., Høgset, A., Bogoeva, V., Slupphaug, G. & Hagen, L. (2023). Proteomic analysis reveals mechanisms underlying increased efficacy of bleomycin by photochemical internalization in bladder cancer cells. Molecular Omics, 19, 585-597.en_US
dc.description.abstractPhotochemical internalization (PCI) is a promising new technology for site-specific drug delivery, developed from photodynamic therapy (PDT). In PCI, light-induced activation of a photosensitizer trapped inside endosomes together with e.g. chemotherapeutics, nucleic acids or immunotoxins, allows cytosolic delivery and enhanced local therapeutic effect. Here we have evaluated the photosensitizer meso-tetraphenyl chlorine disulphonate (TPCS2a/fimaporfin) in a proteome analysis of AY-27 rat bladder cancer cells in combination with the chemotherapeutic drug bleomycin (BML). We find that BLMPCI attenuates oxidative stress responses induced by BLM alone, while concomitantly increasing transcriptional repression and DNA damage responses. BLMPCI also mediates downregulation of bleomycin hydrolase (Blmh), which is responsible for cellular degradation of BLM, as well as several factors known to be involved in fibrotic responses. PCI-mediated delivery might thus allow reduced dosage of BLM and alleviate unwanted side effects from treatment, including pulmonary fibrosis.en_US
dc.publisherRoyal Society of Chemistry (RSC)en_US
dc.rightsNavngivelse-Ikkekommersiell 4.0 Internasjonal*
dc.titleProteomic analysis reveals mechanisms underlying increased efficacy of bleomycin by photochemical internalization in bladder cancer cellsen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2023 The Royal Society of Chemistryen_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470en_US
dc.source.journalMolecular Omicsen_US
dc.relation.projectNorges forskningsråds INFRASTRUKTUR-program: 295910en_US
dc.relation.projectBulgarian National Science Fund: KP-06-H41-9/2021en_US
dc.relation.projectPRIDE and Norstore/Notur projects: NN9036K/NS9036Ken_US
dc.description.localcodePaid open accessen_US

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Navngivelse-Ikkekommersiell 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse-Ikkekommersiell 4.0 Internasjonal